Professor, Pathology and Immunology
Room 7730, CSRB
Office: (314) 362-8730
E-mail: baenziger@wustl.edu
Pub Med Search | |
Research
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| Unique carbohydrate structures have been described on an increasing number of secreted and membrane glycoproteins. Recognition of these structures by highly specific receptors has important biologic consequences. These carbohydrate specific receptors play roles in glycoprotein folding and assembly, inflammation, development, malignant transformation, and targeting. We have identified a new carbohydrate binding motif that mediates recognition of a unique family of carbohydrate structures terminating with the sequence SO4-4-GalNAcâ 1,4GlcNAcâ 1,4Maná (S4GGnM). This receptor has a second, independent binding site for carbohydrates that terminate with mannose (Man). The Man/GalNAc-4-SO4-Receptor is present in a number of different cell types. Its expression is both developmentally and hormonally regulated in a cell specific manner. We are continuing to identify new secreted and membrane glycoproteins that bear oligosaccharides with terminal GalNAc-4-SO4 and are recognized by the Man/GalNAc-4-SO4-Receptor. The Man/GalNAc-4-SO4-Receptor is a multifunctional receptor that is an important model for understanding how unique oligosaccharide structures are utilized for complex biologic processes in vivo. It plays an important role in reproduction in vivo because it recognizes the sulfated carbohydrates on LH, thereby controlling the circulatory half-life of LH. It has different roles in other settings.
The expression of function by receptors like the Man/GalNAc-4-SO4-Receptor requires that unique carbohydrate structures be synthesized on specific glycoproteins. The sulfated carbohydrates on LH are assembled by glycosyltransferases that are protein-specific. Expression of these transferases, like expression of the above receptor, is developmentally and hormonally regulated in a cell specific manner. We are determining at a molecular level how glycosyltransferase expression is regulated during development and during different phases of the hormonal cycle; how expression of the GalNAc-4-SO4-receptor is regulated; and how these structures play a role in recognition events which occur during inflammation and development. Since these carbohydrates and the Man/GalNAc-4-SO4-Receptor play important roles in multiple settings, we are preparing mutant mice deficient in receptor expression to establish the role of this system in vivo. Using these model systems we will define the multiple biologic roles played by this complex system. |
Service to the Department
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| Present | Pathology Course: Lecturer |
| Present | Autopsy Service: Attending |
Service to the University
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| Present | Steering Committee Molecular Cell Biology |
| Present | Admissions Committee DBBS |
| Present | Molecular Cell Biology Course: discussion section |
Clinical Interest
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| Anatomic and Molecular Pathology - Autopsy Pathology |
Selected Publications
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| Woodworth A, Pesheva P, Fiete D, Baenziger JU.. Neuronal specific synthesis and glycosylation of tenascin-R.. J Biol Chem 279:10413-10421, 2004 Abstract
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| Park EI, Manzella SM, Baenziger JU. Rapid clearance of sialylated glycoproteins by the asialoglycoprotein receptor. J Biol Chem 278:4597-4602, 2003 Abstract
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| Baenziger JU. A major step on the road to understanding a unique post-translational modification and its role in a genetic disease.. Cell 113:421-422, 2003 Abstract
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| Baenziger JU.. Glycoprotein hormone GalNAc-4-sulfotransferase.. Biochem Soc Trans 31:326-330, 2003 Abstract
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| Roseman DS, Baenziger JU.. Recognition of carbohydrates in biological systems. The Man/GalNAc-4-SO4-receptor: relating specificity to function.. Methods Enzymol 363:121-133, 2003 Abstract
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| Woodworth A, Fiete D, Baenziger JU.. Spatial and temporal regulation of tenascin-R glycosylation in the cerebellum. J Biol Chem 277:50941-50947, 2002 Abstract
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| Kang HG, Evers MR, Xia G, Baenziger JU, Schachner M.. Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3: A novel member of the HNK-1 family of sulfotransferases.. J Biol Chem 277:34766-34772, 2002 Abstract
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| Mi Y, Shapiro SD, Baenziger JU.. Regulation of lutropin circulatory half-life by the mannose/N-acetylgalactosamine-4-SO4 receptor is critical for implantation in vivo.. J Clin Invest 109:269-276, 2002 Abstract
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| Evers MR, Xia G, Kang HG, Schachner M, Baenziger JU.. Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase.. J Biol Chem 276:36344-36353, 2001 Abstract
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| Roseman DS, Baenziger JU.. The mannose/N-acetylgalactosamine-4-SO4-receptor displays greater specificity for multivalent than monovalent ligands.. J Biol Chem 276:17052-17057, 2001 Abstract
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| Kang HG, Evers MR, Xia G, Baenziger JU, Schachner M.. Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal â 1,4-linked N-acetylgalactosamine.. . J Biol Chem 276:10861-10869, 2001 Abstract
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| Xia G, Evers MR, Kang H-G, Schachner M, Baenziger JU.. Molecular cloning and expression of the pituitary glycoprotein hormone N-acetylgalactosamine-4-O-sulfotransferase.. J Biol Chem 275:38402-38409, 2000 Abstract
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| Keusch J, Manzella SM, Nyame KA, Cummings RD, Baenziger JU.. Cloning of Gb3 synthase, the key enzyme in globo-series glycospingolipid synthesis, predicts a family of á 1,4 glycosyltransferases conserved in plants, insects and mammals.. J Biol Chem 275:25315-25321, 2000 Abstract
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| Keusch J, Manzella SM, Nyame KA, Cummings RD, Baenziger JU.. Expression cloning of a new member of the ABO blood group glycosyltransferases iGb3 synthase, that directs the synthesis of isoglobo-glycosphingolipds.. J Biol Chem 275:25308-25314, 2000 Abstract
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| Roseman DS, Baenziger JU.. Molecular basis of LH recognition by the Man/GalNAc-4-SO4-receptor.. Proc Natl Acad Sci USA 97:9949-9954, 2000 Abstract
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| Fiete D, Beranek MC, Baenziger JU.. The cysteine-rich domain of the "mannose" receptor mediates GalNAc-4-SO4 binding.. Proc Natl Acad Sci USA 95:2089-2093, 1998 Abstract
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| Fiete D, Beranek MC, Baenziger JU.. The macrophage/endothelial cell mannose receptor cDNA encodes a protein which binds oligosaccharides terminating with SO4-4-GalNAcâ1,4GlcNAcâ or Man at independent sites.. Proc Natl Acad Sci USA 94:11256-11261, 1997 Abstract
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| Manzella SM, Dharmesh SM, Cohick CB, Soares MJ, Baenziger JU.. Developmental regulation of pregnancy-specific oligosaccharide structure, NeuAcá2,6GalNAcâ1,4GlcNAc, on select members of the rat prolactin family.. J Biol Chem 272:4775-4782, 1997 Abstract
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| Hooper LV, Manzella SM, Baenziger JU.. The biology of sulfated oligosaccharides. Glycosciences: Status and Perspectives (H.-J. Gabi, 1997
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Current Trainees
| | Lindsey Taylor | | Nicquet Blake, PhD | | Mingqiang Xie, PhD | | Yiling Mi, PhD |
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