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Faculty > Faculty Alphabetical > Douglas Lublin, MD, PhDProfessor, Pathology and Immunology
Director, Clinical Services
Kingshighway Building, 2nd Floor, Room 2439
Office: (314) 362-8847
Lab: (314) 362-8848
E-mail: lublin@wustl.edu
Pub Med Search |  |
Research
| | Our research focuses on cholesterol-rich domains of the plasma membrane termed lipid rafts and caveolae. Rafts are detergent-insoluble domains possessing high levels of cholesterol and sphingolipids, along with a restricted set of membrane proteins. The lipid rafts can be organized by the protein caveolin into pits in the plasma membrane known as caveolae. Evidence implicates the lipid rafts and caveolae in many signal transduction pathways, providing a site for spatially and temporally restricted interactions of signaling molecules.
Our studies focus on three proteins that reside in lipid rafts: src kinases, CD36, and caveolin. (i) The src family of protein tyrosine kinases are involved in multiple signaling pathways, and we have found that raft-resident src kinases have enhanced activity. Ongoing studies are assessing the molecular basis of this effect. (ii) The scavenger receptor CD36 is a major receptor for oxidized low density lipoprotein (oxLDL), and uptake of oxLDL by macrophages leads to formation of foam cells and is a critical early step in the process of atherosclerosis. Our studies are aimed at elucidating how the lipid rafts and caveolae participate in this function of CD36, as well as investigating how the structural features of CD36, including palmitoylation, affect oxLDL uptake and signaling through CD36. (iii) Caveolin, the coat protein of caveolae, binds fatty acids, and we have shown that caveolin can be targeted to the surface of lipid droplets, structures that store neutral lipids in cells, including adipocytes. Based on these findings, we are investigating the role of caveolin in fatty acid uptake and storage in lipid droplets. Overall, our studies seek to define the role of lipid modifications of proteins in their function and to assess the role of lipid rafts and caveolae in cellular processes. |
Diagnostic Expertise
| | Blood Banking and Transfusion Medicine |
Clinical Interest
| | Laboratory and Genomic Medicine - Blood Bank & Transfusion Medicine | | Special Diagnostic Services - Hepatitis Consultation and Reporting | | Special Diagnostic Services - Transfusion Reactions/Hemolytic Anemia |
Selected Publications
| | McOmber D, Ibrahim J, Lublin DM, Saffitz JE, Ong-Simon C, Mendeloff EN, Huddleston CB, Canter CE. Non-ischemic left ventricular dysfunction (NILVD) after pediatric cardiac transplantation: Treatment with plasmapheresis and OKT3. Non-ischemic left ventricular dysfunction (NILVD) after pediatric cardiac transplantation: Treatment with plasmapheresis and OKT3, 2004 Abstract
| | Ostermeyer AG, Ramcharan LT, Zeng Y, Lublin DM, Brown DA. Role of the hydrophobic domain in targeting caveolin-1 to lipid droplets. J Cell Biol 164:69-78, 2004 Abstract
| | Young PP, Uzieblo A, Trulock E, Lublin DM, Goodnough LT. Autoantibody formation after alloimmunization: are blood transfusions a risk factor for autoimmune hemolytic anemia?. Transfusion 44:67-72, 2004 Abstract
| | Zeng Y, Tao N, Chung KN, Heuser JE, Lublin DM. Endocytosis of oxidized low density lipoprotein through scavenger receptor CD36 utilizes a lipid raft pathway that does not require caveolin-1. J Biol Chem 278:45931-45936, 2003 Abstract
| | Lawrence DW, Bruyninckx WJ, Louis NA, Lublin DM, Stahl GL, Parkos CA, Colgan SP. Anti-adhesive role of apical decay-accelerating factor (DAF) in human neutrophil transmigration across mucosal epithelia. J Exp Med 198:999-1010, 2003 Abstract
| | Storry JR, Sausais L, Hue-Roye K, Mudiwa F, Ferrer Z, Blajchman MA, Lublin DM, Ma BW, Miquel JF, Ner. GUTI: a new antigen in the Cromer blood group system. Transfusion 43:340-344, 2003 Abstract
| | Frank PG, Marcel YL, Connelly MA, Lublin DM, Franklin V, Williams DL, Lisanti MP. Stabilization of caveolin-1 by cellular cholesterol and scavenger receptor class B type I. Biochemistry 41:11931-11940, 2002 Abstract
| | Harris CL, Lublin DM, Morgan BP. Efficient generation of monoclonal antibodies for specific protein domains using recombinant immunoglobulin fusion proteins: pitfalls and solutions. J Immunol Methods 268:245-258, 2002 Abstract
| | Lee H, Woodman SE, Engelman JA, Volonte D, Galbiati F, Kaufman HL, Lublin DM, Lisanti MP. Palmitoylation of caveolin-1 at a single site (Cys-156) controls its coupling to the c-Src tyrosine kinase: targeting of dually acylated molecules (GPI-linked, transmembrane, or cytoplasmic) to caveolae effectively uncouples c-Src and caveolin-1 (TYR-14). J Biol Chem 276:35150-35158, 2001 Abstract
| | Ostermeyer AG, Paci JM, Zeng Y, Lublin DM, Munro S, Brown DA. Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets. J Cell Biol 152:1071-1078, 2001 Abstract
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