| Antigens |
Proteins: | 10 - 100 μg per injection, per animal | Cells: | 0.5 - 5.0 x 107 cells per injection, per animal |
| Adjuvant |
| Freund's Adjuvant or Alum |
| Freund's1 |
| Immunization Schedule |
| Day | Manipulation | Adjuvant | Site |
| 0 | Primary Immunization | CFA | s.c. |
| 14 | Boost #1 | IFA | s.c. |
| 28 | Boost #2 | IFA | s.c. |
| 36 | Serum Collection | | |
| 42 | Rest Prior to Fusion (or Boost #3) | IFA | s.c. |
| 56 | i.v. Boost | none | i.v. |
| 59 | Harvest Spleen and Fuse | | |
